Figure 1 from SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte Reprogramming
Year: 2025
Type: other
Abstract: SALL4 is specifically required for YAP1-mediated HC-to-CCA transformation. A, qRT-PCR analysis showing the relative expression levels of Sall4 in different murine liver tumor models [AY-CCA, ANRAS-combined HCC–CCA, KP53-mixed HCC–CCA, and β-catenin (β-cat)-driven HCCs]. B, Schematic representation of the experimental design. HDTVI was used to deliver oncogenes (AY: myrAkt-HA + YAP1S127A; ANRAS: myrAkt-HA + NRASG12V; KP53: KRASG12D+ shp53) into Sall4fl/fl mice, combined with a prior intravenous injection (2 weeks before HDTVI) of AAV8-TBG-GFP (Sall4 WTGFP) or AAV8-TBG-Cre (Sall4 KOhepΔ). Livers were analyzed 4 to 6 weeks after HDTVI. C, Representative liver images from each model (AY, ANRAS, and KP53) comparing Sall4 WTGFP and Sall4 KOhepΔ. D, LW/BW ratio analysis across all models comparing Sall4 WTGFP and Sall4 KOhepΔ (n = 3–4 mice per group). E, IHC for panCK (CC lineage marker) and HNF4A (HC lineage marker). F, Quantification of panCK+ area (%) in ANRAS and KP53 livers. G, IHC for HA-tag and the proliferation marker Ki-67 in AY tumors from Sall4 WTGFP and Sall4 KOhepΔ mice. Data are presented as the mean ± SD; Student t test (**, P < 0.01; ***, P < 0.001; ns, not significant). Scale bars, (E) 200 μm; G, 1 mm (overview), 200 μm (magnified views).
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414528743
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