Figure 5 from SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte Reprogramming
Year: 2025
Type: other
Abstract: SALL4 regulates regenerative HC-to-CC conversion in the DDC-fed cholestasis model. A, Schematic experimental design. Sall4fl/fl mice were injected with either AAV8-TBG-GFP (Sall4 WThep-GFP) or AAV8-TBG-Cre (Sall4 KOhepΔGFP) 2 weeks prior administration of a DDC-containing diet for 4 weeks. Liver tissues were analyzed at the end of the treatment period. B, LW/BW ratio comparison and serum biochemistry analysis comparing alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin levels between Sall4 WThep-GFP and Sall4 KOhepΔGFP groups (n = 4–6 mice per group). C, Representative images of CD45 IHC and Sirius Red staining in livers sections from both groups. D and E, Quantification of (D) CD45+ inflammatory cells per 1,000 μm2 and (E) collagen deposition (%) by Sirius Red staining. F, Representative liver sections stained with hematoxylin and eosin (H&E), SOX9 (CC lineage marker), and Ki-67 (proliferation marker). G and H, Quantification of (G) SOX9+ cells (%) and (H) Ki-67+ cells in the periportal area (%). I, IF staining for SOX9, HNF4A, and GFP. Yellow arrowheads indicate intermediate cells expressing both CC (SOX9) and HC (HNF4A) markers. J, Quantification of SOX9+HNF4A+ double-positive intermediate cells among total HNF4A+ cells (%). K, IF staining for CK19 and GFP to visualize HC-derived CC. L, Quantification of CK19+ CCs per microscopic field. M, Quantification of CK19+GFP+ double-positive cells among total CK19+ cells (%). Data are presented as the mean ± SD; Student t test (*, P < 0.05; **, P < 0.01; ****, P < 0.0001; ns, not significant). Scale bars, (C–F) 100 μm; (K–M) 50 μm.
Language: English
Subfield: Hepatology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W4414529410
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