Figure 4 from SALL4 Is Required for YAP1-Dependent Malignant and Regenerative Hepatocyte-to-Cholangiocyte Reprogramming
Year: 2025
Type: other
Abstract: Bmi1 modulation phenocopied Sall4 modulation in AY-driven HC-to-CCA tumorigenesis. A, qRT-PCR analysis showing Bmi1 expression in AY-Sall4 compared with AY-GFP. B, Schematic experimental design. C and H, Representative macroscopic images of livers harvested from indicated groups. D, LW/BW ratio comparison (n = 3–5 per group). E and I, IHC analysis for indicated markers (HA-tag, HNF4A, Ki-67, panCK, and V5-tag) in experimental groups. F and J, Quantification of Ki-67+ proliferating cells (%) and lipid droplets area per field (%) comparing Bmi1 KOCCAΔ and AY-Bmi1 tumors. G, qRT-PCR analysis showing Bmi1 expression in AY-Bmi1 compared with AY-GFP. Data are presented as the mean ± SD; Student t test (*, P < 0.05; ***, P < 0.001; ****, P < 0.0001). Scale bars, (E and I) 2 mm (overview), 200 μm (magnified views).
Language: English
Subfield: Cell Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414529470
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