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Low-Cost Sucrose Gradient Protocol for GFP Purification from Recombinant E. coli: A Visual Fluorescence-Based Method for Resource-Limited Laboratories v1

Year: 2025

Type: preprint

Abstract: This protocol describes a simple, cost-effective method for purifying Green Fluorescent Protein (GFP) from E. coli using a sucrose gradient. It avoids toxic chemicals and expensive chromatography systems, making it accessible and environmentally friendly. The method preserves GFP's fluorescence and is suitable for applications in biomedical imaging, molecular tagging, and gene expression education laboratories. with further improvements, it could be used in research studies.

DOI: 10.17504/protocols.io.n92ld62pxg5b/v1

Authors: Joseph shenekji, Kamar Shayah, Suad Ali Hassna, Wjoud Kahel, Hind Alnaem, Najwa Hasn Bek

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Responsible consumption and production

Open Access status: gold

OpenAlex ID: W4414531400

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