Work

Generation of a human induced pluripotent stem cell line (SDCHi005-A) from peripheral blood mononuclear cells of a patient with epilepsy

Year: 2025

Type: article

Abstract: Epilepsy is a chronic cerebral disorder characterized by recurrent, episodic, and transient dysfunction of the central nervous system due to abnormal and excessive neuronal activity. In this study, peripheral blood mononuclear cells (PBMCs) were isolated from a young epilepsy patient carrying a DEP domain-containing 5 (DEPDC5) gene nonsense mutation, which was confirmed through clinical and genetic diagnosis. Induced pluripotent stem cells (iPSCs) were established using a non-integrating method carrying plasmids encoding OCT4, SOX2, KLF4, BCL-XL and C-MYC. The constructed iPSCs exhibited typical pluripotent cells morphology, normal karyotype, and demonstrated trilineage differentiation potential.

Source: Stem Cell Research

DOI: 10.1016/j.scr.2025.103839