Inhibition of miR-199b-5p Suppresses the Tuberculosis-Induced Inflammation in Spinal Tuberculosis via Targeting Gcnt2
Year: 2025
Type: article
Abstract: Spinal tuberculosis (STB) is more common in children and young adults and can lead to neurological deficits and spinal deformity in severe cases. Therefore, it is important to find a biomarker for studying the pathogenesis of STB. RT-qPCR was utilized to measure the expression of miR-199b-5p in STB. Moreover, the GPL33147 database was utilized to validate the expression of miR-199b-5p in both STB patients and healthy individuals. Pearson's correlation analysis was employed to explore the correlation between miR-199a-5p and Glucosaminyl (N-acetyl) transferase 2 (Gcnt2) in peripheral blood mononuclear cells (PBMCs) and tissues from STB. The Mycobacterium tuberculosis (Mtb) infection model was constructed by infecting THP-1 cells with 10 multiplicity of infection (MOI) Mtb for 24 h. Overexpressed and inhibited miR-199b-5p and Gcnt2 in the Mtb infection model were realized by cell transfection. ELISA was utilized to measure the expression of inflammatory factors. The target of miR-199b-5p was forecasted by TargetScan. Additionally, the interaction between miR-199b-5p and Gcnt2 was elucidated through the implementation of a dual-luciferase assay system. The expression of miR-199b-5p was increased in PBMCs and tissues from STB, and the two were positively correlated. Gcnt2 was the downstream target of miR-199b-5p, and they negatively correlated in PBMCs and tissues from STB. In the Mtb infection model, overexpressed miR-199b-5p increased inflammatory factors, and overexpression of Gcnt2 reduced this increase. miR-199b-5p was increased in STB, which was a potential biomarker of STB. Notably, miR-199b-5p and Gcnt2 exhibited a collaborative role in STB, displaying an inverse correlation with one another within this context.
Authors: Aierpati Yusufu, Runze Du, Muradil Mardan, Xuyang Xie, Xiaoyu Cai, Tao Xu, Weibin Sheng, Mardan Mamat
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4414536940
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