Work

Lipidomic profiling reveals systemic serum lipid remodeling induced by dexamethasone in mice

Year: 2025

Type: article

Abstract: Background: Dexamethasone is a commonly used glucocorticoid medication in pediatrics. However, dexamethasone is associated with some side effects, such as dyslipidemia. This study aimed to explore the effects of dexamethasone on the serum lipidome. Methods: We utilized Exactive Orbitrap-MS technology to assess the effects of dexamethasone intervention on serum lipids in mice. Results: Unbiased Principal Component Analysis (PCA) revealed that dexamethasone intervention significantly induced changes in serum lipids in mice, and after a 7-day washout period (equivalent to 28 drug half-lives), changes of lipids in the serum were still existed compared with those in the control groups. After 4 days of dexamethasone injection, significant changes were observed, including 16 increased lipids, and 1 decreased lipid in the serum compared with those in the control groups. After a 7-day washout period, some of lipids in the serum were still changed, including 5 increased lipids, such as Acylcarnitines (CAR), ceramides (Cer), diacylglycerophosphates (DG), lysophosphatidylglycerol (LPG) and phosphatidylglycerol (PG), 1 decreased lipid, hexosylceramides (HexCer), indicating dexamethasone may result in long-term changes of lipids in the serum. Discussion: In conclusion, utilizing a lipidomics method, we provide the first complete proof that dexamethasone intervention generates extensive modification of the serum lipidome.

Source: Frontiers in Molecular Biosciences

DOI: 10.3389/fmolb.2025.1644637

Authors: Aiping Zhang, Zhao Wang, Jianjian Ji

Institution: Nanjing University of Chinese Medicine

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801