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Hsa_circ_0075451 promotes NSCLC proliferation, metastasis, and glycolysis through interaction with the RNA-binding protein RBM4

Year: 2025

Type: article

Abstract: OBJECTIVE: To investigate the mechanism of hsa_circ_0075451 in promoting NSCLC proliferation, metastasis, and glycolysis through interaction with RBM4. METHODS: Cancerous tissues and paracancerous tissues were collected from 53 cases of NSCLC. RT-qPCR or Western blot was performed to detect hsa_circ_0075451 and RBM4. Relevant sequences or plasmids were transfected into NSCLC cell line A549, and the malignant phenotype of NSCLC cells was detected by CCK-8, EdU, AnnexinV-PI double staining assay, and Transwell. Glycolysis was assessed by glucose consumption, lactic acid production, and glycolysis-related proteins. In vivo tumorigenesis assays were performed to investigate hsa_circ_0075451 functions in NSCLC. Mechanistic studies were analyzed to verify the interaction of hsa_circ_0075451 and RBM4. RESULTS: Hsa_circ_0075451 levels were elevated in NSCLC, and reducing its expression diminished cell proliferation, metastasis, glycolysis, and tumor expansion. Hsa_circ_0075451 interacted with RBM4. RBM4 down-regulation restricted NSCLC cell proliferation, metastasis, and glycolysis. RBM4 up-regulation opposed the effects of hsa_circ_0075451 knockdown. CONCLUSION: Hsa_circ_0075451 promotes NSCLC proliferation, metastasis and glycolysis by interacting with RBM4.

Source: Discover Oncology

DOI: 10.1007/s12672-025-03330-4

Authors: ChongYu Su, YuXuan Wang, Kang Shi, ZheXin Bai, DaPing Yu, ZhiDong Liu

Institutions: Capital Medical University, Beijing Chest Hospital

Language: English

Topic: Circular RNAs in diseases

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,690