Dielectrophoretic Profiling of Candidozyma auris : the Effect of Glucose on Cellular Polarizability
Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Candidozyma auris is an emerging multidrug-resistant fungal pathogen that poses significant challenges to healthcare systems worldwide. Its ability to persist on surfaces and resist common disinfectants contributes to rapid nosocomial transmission, making early and acute detection crucial for infection control. Conventional culture-based identification methods are time-consuming and lack sensitivity, while molecular techniques are expensive and require specialized equipment and trained personnel. This study explores the use of dielectrophoresis (DEP) for the rapid detection of C. auris by quantifying its dielectric properties using the dielectric single-shell model. Furthermore, since glucose plays a fundamental role in yeast metabolism, including in C. auris, we investigate how glucose metabolism affects its dielectric behavior. Changes in ionic concentrations and enzyme activity induced by glucose metabolism can alter the electrical properties of C. auris cells, making them more responsive to external electric fields. By characterizing these dielectric shifts under glucose-rich and glucose-limited conditions, we aim to develop a DEP-based diagnostic platform for the rapid and label-free detection of C. auris . This approach could provide an effective alternative to current diagnostic methods, enhancing screening efforts and improving infection control in healthcare settings.
Source: ACS Measurement Science Au
Authors: Negar Farhang-Doost, Camila S. Cué Royo, Tagbo H. R. Niepa, Soumya K. Srivastava
Institutions: West Virginia University, Carnegie Mellon University
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: gold
Estimated APC paid: $3,500
Funders: National Science Foundation, University of Pittsburgh, Carnegie Mellon University, West Virginia University, National Institutes of Health, National Institute of Allergy and Infectious Diseases, National Institute of Dental and Craniofacial Research, Division of Civil, Mechanical and Manufacturing Innovation
OpenAlex ID: W4414556170