Work

To Investigate the Antibacterial and Antibiofilm Activity of the Antimicrobial Peptide FP-CATH against Carbapenem-Resistant Klebsiella pneumoniae

Year: 2025

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide In light of the increasing prevalence of carbapenem-resistant Klebsiella pneumoniae (CRKP), which represents a significant public health threat, it is imperative to investigate novel therapeutic strategies. This study aims to assess the efficacy of the novel antimicrobial peptide FP-CATH against CRKP, elucidate its potential mechanism of action, evaluate its safety profile and influencing factors, and determine whether FP-CATH possesses anti-inflammatory properties. A total of 15 CRKP strains (comprising 6 KPC2, 6 NDM-1, and 3 KPC2 + NDM-1) and 8 carbapenem-susceptible K. pneumoniae (CSKP) strains were selected for testing. The broth microdilution method was employed to ascertain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of FP-CATH against these strains. The ability of FP-CATH to disrupt established CRKP biofilms was assessed by using crystal violet staining. The mechanism of action of FP-CATH was investigated by using NPN and PI fluorescent probes. Serum coincubation experiments were conducted to evaluate the serum stability of FP-CATH. Additionally, RT-qPCR was utilized to measure the expression of inflammatory factor genes in bacterially infected cells. Finally, the efficacy of FP-CATH was validated in vivo using a Galleria mellonella infection model.

Source: ACS Omega

DOI: 10.1021/acsomega.5c05443

Author: Lipeng Zhong

Institutions: Nanchang University, First Affiliated Hospital of Jiangxi Medical College, First Affiliated Hospital of Nanchang University

Language: English

Topic: Antimicrobial Peptides and Activities

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,935