Spatiotemporal Differences of GABAergic Polarization and Shunting During Dendritic Integration
Year: 2025
Type: article
Abstract: ABSTRACT Aim In the adult brain, GABA exerts either depolarizing or hyperpolarizing effects on neuronal membranes, depending on neuron type, subcellular location, and neuronal activity. Depolarizing GABA typically inhibits neurons through shunting, which is mediated by increased membrane conductance upon GABA A receptor activation; however, it can also excite neurons by recruiting voltage‐dependent conductances. The net influence of these opposing actions of depolarizing GABA on glutamatergic synaptic inputs remains incompletely understood. We aimed to examine the spatiotemporal characteristics of membrane polarization and shunting mediated by GABA A receptors and assess their functional impact on the integration of GABAergic and glutamatergic inputs along dendrites. Methods Using whole‐cell current‐clamp recordings in CA1 pyramidal neurons and dentate gyrus granule cells (GCs) from rat hippocampal slices, we mimicked GABAergic and glutamatergic inputs with local GABA puff and glutamate spot‐uncaging, respectively. A mathematical model further quantified the relative effects of local shunting and polarization. Results Depolarizing GABAergic postsynaptic responses (GPSRs) exhibited biphasic actions, exerting inhibitory effects at the synapse through shunting, and excitatory effects distally, where depolarization predominated. The excitatory component also persisted longer than the shunting inhibition. In contrast, hyperpolarizing GPSRs remained consistently inhibitory across both spatial and temporal dimensions. Conclusions These findings highlight the complex spatiotemporal interplay between shunting and membrane polarization mediated by GABAergic inputs, providing new insights into dendritic computation and neuronal network dynamics.
Source: Acta Physiologica
DOI: 10.1111/apha.70112
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4414560715