Effects of 2-bromoethanesulfonate alone or in combination with calcium propionate or monensin on methane and gaseous hydrogen production during in vitro rumen fermentation
Year: 2025
Type: article
Abstract: Some feed additives that mitigate enteric methane (CH 4 ) production can result in an increase in hydrogen (H 2 ) production. When expelled to the environment, H 2 may intensify competition for hydroxyl radicals, prolonging CH 4 persistence in the atmosphere. Therefore, the objective of the experiment was to determine the effects of adding 2-bromoethanesulfonate (BE) alone and in combination with monensin or calcium propionate on in vitro ruminal CH 4 and H 2 production. Using 3 separate runs a 1 g 50% corn silage and 50% alfalfa (DM basis) were fermented 1) alone (CON); 2) with additonal 100 μM BE (BES); 3) BES + additional 2.5 μM monensin (BM); and 4) BES + additional 2.5% DM calcium propionate (BC). Inoculum was obtained by collecting a mixture of rumen fluid from 2 steers (BW = 657 ± 12.5 kg) consuming a diet of 30% concentrate and 70% roughage. For each treatment within run, gas production was measured over a 48-h period using 2 randomly assigned paired and separate modules. Total CH 4 and H 2 production was calculated by multiplying total volume of gas produced by the concentration of CH 4 and H 2 determined by gas chromatography at 0, 4, 8, 18, 24, and 48 h. Data were blocked by run and analyzed as a repeated measures randomized complete block design using the GLIMMIX procedure of SAS (9.4) where treatment, time and the interaction between these 2 factors were considered as fixed effects while module, run and the interaction were included as random effects. Significance was declared at P ≤ 0.05. The BES, BM, and BC treatments did not differ in CH 4 production (g/h) at 4 and 8 h of incubation averaging 0.005 and 0.013 ± 0.0020 g/h respectively, but compared with the CON (0.017 and 0.047 ± 0.0018 g/h CH 4 , respectively) was reduced. At 8 h BES increased H 2 production relative to BM and BC (0.002 and 0.0014 ± 0.0002 g/h H 2 , respectively), but all treatments containing BES were significantly greater than CON (0 vs 0.002 ± 0.0002 H 2 g/h).). In conclusion, treatments containing BES reduced CH 4 , and the addition of calcium propionate or monensin may serve as a short-term strategy for the reduction of H 2 resulting from mitigation of enteric CH4 production.
Source: JDS Communications
Authors: B. Rinn, A.K. Neff, G. Fincham, P.J. Kononoff, A.L. Carroll
Institutions: University of Nebraska–Lincoln, Washington State University
Language: English
Subfield: Agronomy and Crop Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $1,310
Award: G2999968413
OpenAlex ID: W4414571899