Dendrimer-based DNA biosensor for HIV virus detection
Year: 2025
Type: article
Abstract: In this work, we propose an innovative electrochemical DNA biosensor for the early, rapid, selective, and sensitive detection of the human immunodeficiency virus (HIV) based on its genetic code. This platform is based on the integration of few-layer bismuthene (FLB) which provides a nanostructured surface and facilitates the anchoring of the DNA dendrimer which incorporates the biorecognition element (a capture probe complementary to the HIV-specific DNA sequence). The hybridization between the capture probe and the specific HIV target sequence is detected using Azure A (AA) as a redox indicator. The dendrimer-based DNA biosensor selectively detects HIV from 10.0 fM to 10.0 pM, exhibit a low detection limit of 3.03 fM and a 60-day stability, and has been validated using plasma samples from infected patients with varying viral loads.
Source: Biosensors and Bioelectronics
Institutions: Madrid Institute for Advanced Studies, IMDEA Nanoscience, Universidad Autónoma de Madrid, Institute of Micro and Nanotechnology, Consejo Superior de Investigaciones Científicas, Centro de Biología Molecular Severo Ochoa, Hospital Universitario La Paz, Centro de Investigación Biomédica en Red Enfermedades Infecciosas
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,540
Awards: G1050946522, G1105844351, G1175115578, G121809054, G1446647517, G1610934229, G1855990336, G210041235, G2221335197, G2262748287, G2309174360, G2380629619, G2601397997, G2660406951, G279363373, G2832855986, G300979063, G3109769530, G34346518, G3480869486, G352902418, G359587020, G3681454997, G4015142043, G4042783231, G4052934924, G425668148, G430777504, G4418367529, G4717959651, G479515186, G488842982, G491712853, G503093362, G5080475149, G5101445077, G5195188938, G524206111, G5283148391, G5404395142, G5421974903, G5428008381, G5690961148, G6143908864, G624877701, G6959824596, G7084143925, G730288178, G7616658214, G7805626310, G7863371703, G819325146, Magnetoelectric 3D printing technology - the revolution of actuatable composites, G8434830731, G8497050386, G8845005669, G8920660035, G897484643, G975344006, G99296377
OpenAlex ID: W4414573559