Work

Synergistic effect of plant-derived deglucoruscin in combination with conventional antimicrobials against Staphylococcus aureus and Candida albicans

Year: 2025

Type: article

Abstract: A detailed phytochemical investigation of rhizomes and roots of Ruscus aculeatus led to the isolation of fifteen pure compounds, belonging to the furostanol and spirostanol glycoside classes . Among them, one novel furostanol saponin ( 1 ) was identified, alongside twelve known furostanol ( 2 – 8 ) and spirostanol ( 11 – 15 ) saponins and two sulphated glycosides ( 9 – 10 ). Structural elucidation of all isolated compounds was accomplished through comprehensive 1D and 2D NMR spectroscopic analyses, complemented by mass spectrometry. The CHCl 3 extract of Ruscus aculeatus and its major metabolite, the spirostanol derivative ( 12 ), exhibited a weak antimicrobial activity. Of particular interest, compound 12 when co-administered with voriconazole (VOR) showed a significant inhibitory effect against the azole-resistant Candida albicans ATCC 10231 strain, demonstrating a synergistic effect that underscores its potential to restore antifungal efficacy and combat resistance mechanisms. These findings highlight compound 12 as a promising adjuvant candidate in antifungal therapy, particularly against resistant Candida strains.

Source: Fitoterapia

DOI: 10.1016/j.fitote.2025.106895