Work

Activin receptor type IIA/IIB blockade increases muscle mass and strength, but compromises glycemic control in mice

Year: 2025

Type: article

Abstract: Blocking the Activin receptor type IIA and B (ActRIIA/IIB) has clinical potential to increase muscle mass and improve glycemic control in obesity, cancer, and aging. However, the impact of blocking ActRIIA/IIB on strength, metabolic regulation, and insulin action remains unclear. Here, we investigated the effect of short- (10mg kg -1 bw, once, 40h) or long-term (10mg kg -1 bw, twice weekly, 21 days) antibody treatment targeting ActRIIA/IIB (αActRIIA/IIB) in lean and diet-induced obese mice and engineered human muscle tissue. Short-term ActRIIA/IIB administration in lean mice increased insulin-stimulated glucose uptake in skeletal muscle by 76-105%. Despite this, ActRIIA/IIB-treated mice exhibited 33% elevated blood glucose and glucose intolerance. Long-term αActRIIA/IIB treatment increased muscle mass (+20%) and reduced fat mass (–8%) in obese mice but failed to enhance insulin-stimulated glucose uptake in muscle or adipose tissue. Instead, it induced glucose intolerance, cardiac hypertrophy with glycogen accumulation, and elevated hepatic triacylglycerol and glucose output in response to pyruvate. Concomitantly, long-term ActRIIA/IIB treatment increased strength (30%) in mouse soleus muscle and prevented activin A-induced loss of tissue strength in engineered human muscle tissue. Surprisingly, long-term ActRIIA/IIB treatment lowered volitional running (-250%). Our findings demonstrate that, in accordance with human studies, ActRIIA/IIB blockade holds promise for increasing muscle mass, strength, and muscle insulin sensitivity. However, contrary to the improved glycemic control in humans, ActRIIA/IIB blockade in mice causes severe glucose intolerance and lowers voluntary physical activity. Our study underscores the complex metabolic and functional consequences of ActRIIA/IIB blockade, and highlight species differences on glycemic control, which warrant further investigation. • ActRIIA/IIB blockade enhances insulin-stimulated glucose uptake. • αActRIIA/IIB treatment increases force in mouse muscle and engineered human muscle. • αActRIIA/IIB-treated mice demonstrate reduced voluntary running activity. • αActRIIA/IIB treatment impairs glycemic control and elevates liver triacylglycerol. • Species differences in ActRIIA/IIB targeting are critical for preclinical research.

Source: Molecular Metabolism

DOI: 10.1016/j.molmet.2025.102261