Work

Synthesis, duplex-forming properties, and enzymatic resistance of oligonucleotides containing N -acylsulfonamide linkages

Year: 2025

Type: article

Abstract: the sulfo-click reaction and its incorporation into DNA oligonucleotides. Thermal denaturation studies indicate modest destabilization at duplex ends (∼1 °C) and stronger effects in the middle (∼7 °C), while the NAC linkage provides complete resistance to nucleases.

Source: Organic & Biomolecular Chemistry

DOI: 10.1039/d5ob01382h

Authors: Romain Amador, Carine Baraguey, Guillaume Clavé, Michael Smietana

Institutions: École Nationale Supérieure de Chimie de Montpellier, Centre National de la Recherche Scientifique, Université de Montpellier, Institut des Biomolécules Max Mousseron

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: Agence Nationale de la Recherche

Awards: G3010527862, Thioacid / Azide Ligation Applied to Nucleic acids as a new tool for chemical biology