Work

Visible Light Induced Mukaiyama Reagent Promoted Desulfurative Modification of Peptides and Proteins with Nucleotides

Year: 2025

Type: article

Abstract: -alkyl-2-halopyridinium) activation under visible light (400 or 420-430 nm), this method generates configurationally stable Ala radicals while avoiding the detrimental side effects associated with UVA irradiation. The disulfide-compatible system preserves native stereochemistry and accommodates diverse substrates including oligonucleotides, functionalized nucleosides, and drug conjugates in good yields. Biocompatible reductants (NADH/Hantzsch ester) further facilitate conjugation with various radical acceptors under mild conditions. This approach established a versatile platform that enables both precision modification of peptides/proteins and investigation of structure-function relationships in peptides, proteins, and nucleic acids under physiologically relevant conditions.

Source: ACS Central Science

DOI: 10.1021/acscentsci.5c01241