支持细胞中的CK1α对小鼠睾丸发育和精子发生至关重要
Year: 2025
Type: article
Abstract: Male infertility constitutes a major global public health concern, with the underlying etiology remaining unidentified in nearly half of the diagnosed cases. Protein kinase CK1α (CK1α) functions as a pivotal regulator of cell cycle progression, pre-mRNA processing, and spliceosome-associated pathways through interactions with specific splicing factors. Comprehensive analyses revealed CK1α expression in both germ cells and somatic cells of mouse testes, implicating its involvement in spermatogenic regulation. However, the physiological roles and mechanistic basis of CK1α function in Sertoli cells remain unclear. In this study, CK1α was highly expressed in Sertoli cells, and conditional knockout of CK1α in murine Sertoli cells induced profound testicular atrophy and complete infertility. This phenotype was characterized by rapid depletion of Sertoli cells and spermatogenic dysfunction. Subsequent analyses demonstrated that CK1α regulated the fate determination of fetal and neonatal Sertoli cells in mice. At the molecular level, CK1α promoted Sertoli cell survival through interaction with the splicing factor ZRSR1 to modulate apoptosis. Collectively, these findings establish CK1α as a key regulator of alternative splicing and male reproduction, providing critical insights into the molecular mechanisms underlying testicular development and reproductive function.
Source: 动物学研究
Authors: Guo Hong-Zhou, 扬州大学动物医学院, 江苏 扬州225009, 中国, Wang Ming-Yue, Zhang Di, Zhang Jing-Lin, Zhou Ye-Wen, Gou Ke-Mian, Liu Jia-Li, Liu Zong-Ping, Cui Sheng, 中国农业大学生物学院农用动物生物技术育种国家重点实验室, 北京100193, 中国, 扬州大学农业与农产品安全教育部国际联合实验室, 江苏 扬州225009, 中国, 江苏省重大动物传染病与人畜共患病预防控制协同创新中心, 江苏 扬州225009, 中国
Institutions: Yangzhou University, China Agricultural University
Language: English
Subfield: Reproductive Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W4414625149
FWCI:
Cites:
Cited by:
Related to: