Work

Ca 2+ signal dynamics in maturing ureteric bud- and collecting duct-derived organoid tubules

Year: 2025

Type: article

Abstract: This investigation, focused on analyzing the role of PIEZO1 mechanotransduction in maturing human induced pluripotent stem cell (iPSC)-derived ureteric bud (UB) and collecting duct (CD) kidney organoids, unexpectedly reveals developmentally regulated Ca 2+ oscillations and begins to dissect their mechanistic underpinnings. Specifically, transcriptomic analysis reveals that with time in culture and differentiation from UB to CD, organoids progressively acquire the molecular machinery necessary for complex Ca 2+ signaling dynamics. These results lead us to speculate that information encoded in oscillatory signals drives renal epithelial differentiation.

Source: American Journal of Physiology-Cell Physiology

DOI: 10.1152/ajpcell.00578.2025

Authors: Rolando Carrisoza-Gaytán, Abigail Daily, Kayla J. Wolf, Samia Lasaad, Anna Cantalupo, Pooja Nair, Ronald C. van Gaal, Per Uhlén, Jennifer A. Lewis, Lisa M. Satlin

Institutions: Icahn School of Medicine at Mount Sinai, Cornell University, Harvard University, Wyss Institute for Biologically Inspired Engineering, Karolinska Institutet, Harvard Stem Cell Institute

Language: English

Topic: Ion Channels and Receptors

Subfield: Sensory Systems

Field: Neuroscience

Domain: Life Sciences

Open Access status: hybrid

Funders: Nederlandse Organisatie voor Wetenschappelijk Onderzoek, Vetenskapsrådet, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: G21510684, Ex Vivo Generation of Functional Kidney Tissues for Transplantation, G3338985746, G577709641, Engineering scalable collecting duct networks for functional kidney tissue, Pittsburgh Center for Kidney Research, Exploration of Intratumoral Heterogeneity with Multimodal 3D Imaging at Single-Cell Resolution to Improve Precision Medicine for Cancer, Role of piezo channels in intercalated cells

OpenAlex ID: W4414645618

FWCI: 0.569

Cites: 92

Cited by: 1

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