Untargeted metabolomics of Artemisia selengensis Turcz leaves and its mechanism of action in improving cholesterol metabolism in high-fat diet-fed rats
Year: 2025
Type: article
Abstract: BACKGROUND/OBJECTIVES: Hyperlipidemia is a lipid metabolic disease and statins are mainly used to treat hyperlipidemia in the clinics.However, resistance to statin therapy is a limitation.Artemisia selengensis Turcz (AST) is a vegetable that has had poorly studied effects on hyperlipidemia. MATERIALS/METHODS:We prepare the AST as a source of various health-promoting nutrients.First, using the root, stem, and leaves of AST were separated and demonstrated its effect on lipid-lowering indices in vitro.Next, we studied the effect of AST leaf in vivo.AST leaf (100 mg/kg BW/d) was used to treat 4-week-old high-fat diet induced male Wistar rats (n = 12) for 21 days. RESULTS:We have observed that anti-hyperlipidemia index were significantly improved by AST leaf in vitro experiment.Moreover, it is significantly ameliorated the serum lipid profile and reduced total cholesterol after 21 days of treatment.mRNA expression of the metabolism-related genes LDLR, CYP7A1, HMGCR, SREBP2, and ABCA1 was also significantly increased.Moreover, we also estimated the feces bile acid level, which increased significantly following treatment with AST in the leaves.Furthermore, phenol and flavonoid levels were significantly greater in the AST leaves (ASTL) than in the roots and stems, as revealed by the untargeted metabolomics analysis. CONCLUSION:The findings revealed that the phenols and flavonoids in ASTL may have contributed to their ability to effectively facilitate hyperlipidemia through the liver-intestinal axis in vivo.
Source: Nutrition Research and Practice
Authors: Cong Liu, Yinxiang Bao, Yi Sun, Darifu Ba, Fan Zhang, Jilite Wang
Institution: Hetao College
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Awards: 新型米糠活性肽调控胆固醇代谢作用机制的研究, G7913359145
OpenAlex ID: W4414737681