Impact of Acute Myeloid Leukemia-Derived Extracellular Vesicles on Expression of CTNNB1, TGF-β, and VEGF Genes in Bone Marrow Mesenchymal Stem Cells: Insights into Leukemia Pathogenesis.
Year: 2025
Type: article
Abstract: Objective: genes, which are pivotal in leukemia progression. Materials and Methods: In this experimental study, AML-derived EVs were isolated from 30 newly diagnosed non-M3 AML patients and characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), Bradford assay, and flow cytometry. BMSCs were isolated from healthy BM aspirates and were co-cultured with EVs at concentrations of 10, 40, and 60 μg/ml for 24, 48, and 72 hours. Outcomes were assessed using quantitative reverse transcription polymerase chain reaction (RT-PCR), (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliuom bromide (MTT) assay, flow cytometry [for apoptosis, reactive oxygen species (ROS), and Ki67], and Western blot analyses. Results: gene expression in BMSCs via the Wnt/β-catenin signaling pathway, as confirmed by increased β-catenin protein levels. However, a 60 μg/ml dose increased apoptosis and reduced gene expression. Conclusion: These findings can suggest that AML-derived EVs modulate the BME by promoting BMSC survival and upregulating pro-leukemic genes at an optimal dose, offering a potential therapeutic target for AML treatment.
Source: PubMed
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4414751534
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