Work

SNHG16 promotes breast cancer progression by regulating the hsa-let-7b-5p/AURKA axis

Year: 2025

Type: article

Abstract: Breast cancer (BC) is the most common cancer among women and ranks as the second leading cause of cancer-related deaths globally. There is a noticeable trend towards younger age groups being affected. Long non-coding RNAs (lncRNAs) play crucial roles in tumor progression. This study focuses on lncRNA SNHG16 and its regulatory role in breast cancer progression, validated through microarray analysis and in vitro experiments.Initially, we identified differential genes in breast cancer from TCGA and GEO databases. By intersecting these datasets, significant differentially expressed genes were identified, followed by enrichment analysis to understand their cellular functions. Bioinformatics predictions guided construction of a competitive endogenous RNA (ceRNA) regulatory network SNHG16-hsa-let-7b-5p/AURKA.Subsequently, the expression of SNHG16 was validated in breast cancer cell lines using quantitative real-time PCR (qRT-PCR) and western blot (WB) experiments. Further investigation involved siRNA-mediated knockdown of SNHG16 to observe downstream gene and protein changes. Functional assays including cell counting (CCK-8) and transwell assays were employed to assess the impact of the SNHG16 on breast cancer cell proliferation and invasion through this ceRNA axis.Our findings underscore the significant regulatory role of SNHG16 in breast cancer development, revealing critical networks associated with high invasiveness and metastasis. These insights provide potential novel targets for breast cancer therapy, offering new avenues for intervention.

Source: Biochemistry and Biophysics Reports

DOI: 10.1016/j.bbrep.2025.102271