Work

Metallo-chlorophyll derivatives as food colorant: Intact chloroplasts from spinach leaf recovered by enzyme-assisted extraction

Year: 2025

Type: article

Abstract: This study presents tailored enzyme-assisted extraction (EAE) to recover intact chloroplasts from spinach, using a synergistic combination of metal ions, in comparison to traditional chlorophyll (Chl) solvent extraction. The content of pigment in intact chloroplasts recovered by EAE is affected by time and metal dose; the highest concentration is achieved using 165 ppm of metals for 2 h. With regard to solvent extraction, the amount of intact chloroplasts is remarkably lower and affected only by process time. Colorimetric analysis confirms a good correlation between the concentration of metal ions and greenness. Applied to meringues, the Chl-based extract (<3% w/w) enhances color without affecting flavor, fragrance, and mouthfeel.

Source: Italian Journal of Food Science

DOI: 10.15586/ijfs.v37i4.3028

Authors: Caterina Mazzocchi, Claudio Lombardelli, Ilaria Benucci, Marco Esti

Institution: Università degli Studi della Tuscia

Language: English

Topic: Botanical Research and Applications

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: gold

Estimated APC paid: $1,100