Year: 2025
Type: preprint
Abstract: This document describes the preparation of extraction buffer stock solution for ancient DNA extraction from bones, teeth, sediments and other materials, following the method described by Dabney et al. 2013 and refined by Rohland et al. 2018. To obtain the final extraction buffer, proteinase K solution (see separate document) must be added on the day of extraction. References Dabney, J., Knapp, M., Glocke, I., Gansauge, M.-T., Weihmann, A., Nickel, B., Valdiosera, C., García, N., Pääbo, S., Arsuaga, J.-L., & Meyer, M. (2013). Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments. Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15758-15763. Rohland, N., Glocke, I., Ayinuer-Petri, A., & Meyer, M. (2018). Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing. Nature Protocols, 13, 2447-2461.
Authors: Anna Schmidt, Sarah Nagel
Institution: Max Planck Institute for Evolutionary Anthropology
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414764505
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