Year: 2025
Type: preprint
Abstract: This document describes the preparation of proteinase K solution for ancient DNA extraction from bones, teeth, sediments and other materials, following the method described by Dabney et al. 2013 and refined by Rohland et al. 2018. Proteinase K is added to the extraction buffer stock solution (see separate document) on the day of DNA extraction. References Dabney, J., Knapp, M., Glocke, I., Gansauge, M.-T., Weihmann, A., Nickel, B., Valdiosera, C., García, N., Pääbo, S., Arsuaga, J.-L., & Meyer, M. (2013). Complete mitochondrial genome sequence of a Middle Pleistocene cave bear reconstructed from ultrashort DNA fragments. Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15758-15763. Rohland, N., Glocke, I., Ayinuer-Petri, A., & Meyer, M. (2018). Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing. Nature Protocols, 13, 2447-2461.
Authors: Anna Schmidt, Sarah Nagel
Institution: Max Planck Institute for Evolutionary Anthropology
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W4414764692
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