Rosa moschata leaf extract ameliorates Alzheimer’s disease in AlCl3 + D-galactose inducedAlzheimer’s rat model via modulation of neuroinflammatory biomarkers and suppression of oxidative stress markers
Year: 2025
Type: article
Abstract: Conventional treatment strategies provide symptomatic relief from Alzheimer’s disease (AD), but their long-term use is associated with the progression of neuronal degeneration. Considering the previously reported in vitro neuroprotective activity of Rosa moschata, this study was designed to evaluate the anti-Alzheimer’s potential of R. moschata leaf extract (RMLE) at safe doses in an aluminum chloride + D-galactose-induced AD rat model. An HPLC/ESI-MS analysis of the RMLE was performed to identify the neuroprotective phytochemicals. An oral acute toxicity study was conducted to determine the safe dosage levels. For the development of the AD model, aluminum chloride + D-galactose (150 mg/kg each) were administered simultaneously to rats, except the control group. RMLE at 50, 100, and 150 mg/kg was administered orally to the treatment groups for three weeks. Neurobehavioral studies were performed, and the levels of antioxidant enzymes and neurotransmitters in the brain homogenate were assessed. Using qPCR, the mRNA expression of neuropathological biomarkers of AD was estimated. HPLC/ESI-MS analysis revealed the presence of 12 compounds in negative mode and 2 com-pounds in positive ionic mode, exhibiting antioxidant and neuroprotective potential. RMLE improved rat behavioral parameters in a dose-dependent manner through the inhibition of oxidative stress and acetylcholinesterase. Neurotransmitter levels and gene expression of neurodegenerative and neuroinflammatory biomarkers (IL-1β, IL-1α, TNF-α, ABPP, β-secretase, and α-synuclein) were significantly restored (P < 0.05) in a dose-dependent manner by RMLE. These findings support the therapeutic application of extracts obtained from R. moschata leaves in neurodegeneration, particularly in AD.
Source: Italian Journal of Food Science
Authors: Norah K. Algarzae, Uzma Saleem, Ifat Alsharif, Rabeea Safdar, Zunera Chauhdary, Moneerah J. Alqahtani, Jawaher Alqahtani, Fatima A. Jaber, Tourki A. S. Baokbah, Reem Hasaballah Alhasani, Tasahil S. Albishi, Sultan F. Kadasah, Sultan M. Alshahrani, Nada M. Mostafa, Omayma A. Eldahshan, Agustina Lulustyaningati Nurul Aminin, Muhammad Ajmal Shah, Rana O. Khayat, Aishah E. Albalawi, Ana Sanches Silva
Institutions: King Saud University, University of the Punjab, Umm al-Qura University, Government College University, Faisalabad, University of Jeddah, King Salman Center for Disability Research, University of Bisha, King Khalid University, Ain Shams University, Diponegoro University, Hazara University, University of Tabuk, University of Lisbon, Universidade do Porto, Centro de Estudos de Ciencia Animal, University of Coimbra
Language: English
Subfield: Complementary and alternative medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $1,100
Award: G2088887155
OpenAlex ID: W4414766591
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