A Precise TaqMan-Based Real-Time qPCR Assay for Detecting and Quantifying Blackberry Chlorotic Ringspot Virus, Blueberry Shock Virus, and Plum Pox Virus in Fruit Tree Seedlings
Year: 2025
Type: article
Abstract: We developed a rapid and efficient TaqMan-based realtime reverse transcription quantitative PCR (RT-qPCR) assay for the detection and quantification of viruses infecting fruit trees, including blackberry chlorotic ringspot virus (BCRV), blueberry shock virus (BlShV), and plum pox virus (PPV). The detection limits for each virus were 40 copies (BCRV), 500 copies (BlShV), and 40 copies (PPV), respectively. Two primer-probe sets were selected for each virus, with amplification efficiencies ranging from 90-110%. High specificity was confirmed against other viruses or viroids sharing the same host plants. Multiplex detection of BCRV, BlShV, and PPV was achieved by using FAM and Cy5 fluorescent dyes. All sets maintained high efficiency and sensitivity with varying amounts of RNA extracted from the woody branches of the host plant. This assay will be useful for rapid and accurate diagnosis of plant virus diseases, especially in quarantine stations where leaf tissue is often unavailable upon import.
Source: The Plant Pathology Journal
Authors: Na Hee Kim, Minhue Jung, Seung Hyeon Oh, Kook-Hyung Kim
Institution: Seoul National University
Language: English
Topic: Plant Virus Research Studies
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $850
Awards: G200041619, G3042804765, G5089324269
OpenAlex ID: W4414780721