Work

DNA/RNA extraction and cleanup protocol for PCR and tag seq applications with coral samples v1

Year: 2025

Type: preprint

Abstract: The extraction of high-quality genomic material from corals can be difficult due to high concentrations of lipids and carbohydrates in coral tissues. This is especially the case for some corals in the genus Porites. This protocol uses a modified PCA protocol to extract combined DNA and RNA from coral tissues stored in 100% ethanol and frozen. The DNA can be diluted and used in varied PCR applications. If the end goal is RNA sequencing, the RNA cleanup protocol can be followed to acquire clean high-quality RNA.

DOI: 10.17504/protocols.io.261gek93jg47/v1

Authors: Carsten Grupstra, Ally Swank, Sarah W. Davies

Institutions: Boston University, Florida Atlantic University

Language: English

Topic: Coral and Marine Ecosystems Studies

Subfield: Ecology

Field: Environmental Science

Domain: Physical Sciences

Open Access status: gold

Funder: National Science Foundation

Award: Collaborative Research: How do selection, plasticity, and dispersal interact to determine coral success in warmer and more variable environments?

OpenAlex ID: W4414797276

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