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In vitro Activity of the Novel Tetracyclines Derivative, Zifanocycline Against Mycobacterium abscessus

Year: 2025

Type: article

Abstract: Objective: This study aimed to evaluate the in vitro antibacterial activity of zifanocycline against Mycobacterium abscessus . Methods: From 2019 to 2024, Mycobacterium abscessus isolates from the respiratory tract of patients were collected at a tertiary hospital in Jiaxing City. All isolates were identified for species, subtypes, and the erm 41 gene polymorphism using whole-genome sequencing (WGS). Susceptibility to zifanocycline and 13 comparators was tested using the broth microdilution method, and the combined effects of zifanocycline and seven antibacterial drugs were evaluated. Results: A total of 67 strains of Mycobacterium abscessus were collected and subjected to whole-genome sequencing. Genomic analysis identified 60 strains of Mycobacterium abscessus subsp. abscessus and 7 strains of Mycobacterium abscessus subsp. massiliense . Among the Mycobacterium abscessus subsp. abscessus strains, 57 exhibited the erm41 T28 genotype, whereas the remaining three showed the erm41 C28 genotype. In our study, zifanocycline (MIC 50 /MIC 90 , 0.06/0.25 mg/L) demonstrated a 2-fold lower MIC 50 and MIC 90 compared to tigecycline (MIC 50 /MIC 90 , 0.12/0.5 mg/L), and a 4-fold and 2-fold lower MIC 50 and MIC 90 , respectively, compared to omadacycline (MIC 50 /MIC 90 , 0.25/0.5 mg/L). In addition to amikacin and linezolid, zifanocycline demonstrated significantly superior antibacterial activity compared with ciprofloxacin, moxifloxacin, trimethoprim–sulfamethoxazole, cefoxitin, doxycycline, imipenem, and clarithromycin. The combination of zifanocycline and the seven antibacterial drugs used for the treatment of Mycobacterium abscessus showed no significant interactions. Conclusion: Zifanocycline exhibits positive antibacterial activity against Mycobacterium abscessus in vitro and has potential as an alternative agent in multidrug combination therapy regimens for the treatment of this pathogen. Keywords: Mycobacterium abscessus , in vitro, tetracycline, zifanocycline

Source: Infection and Drug Resistance

DOI: 10.2147/idr.s538789