Work

Stop Evolution v1

Year: 2025
Type: preprint
Abstract: Terminate the rhamnose-induced high-mutation-rate evolution phase by replacing the culture medium to remove the inducer, thereby shutting down expression of the mutator DNA polymerase in the OrthoRep system. This allows stabilization and selection of target mutant strains for subsequent phenotypic analysis.
Language: English
Subfield: Genetics
Open Access status: gold
OpenAlex ID: W4414827488
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