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PSII-28 Effects of Saccharomyces cerevisiae fermentation product on rumen metabolome of steers fed high grain diet.

Year: 2025
Type: article
Abstract: Saccharomyces cerevisiae fermentation product (SCFP) has been reported to improve feed efficiency, and enhanced the ruminal microbial protein production in some previous studies. However, the mode of action of SCFP in high grain diet is not fully understood. The objective of this study is to investigate the impact of SCFP on rumen metabolome and the correlations between the rumen and plasma metabolites in steers fed high grain diet. A crossover study was conducted using eight rumen cannulated Holstein steers (BW: 467 ± 13.9 kg) over two 21-day periods, with a 7-day washout period in between periods. Steers were stratified based on BW and randomly assigned to one of the two treatments; 1) Control (CON), a basal diet without additive, and 2) SCFP, 12 g/d, top-dressed on the basal diet. Blood samples were collected on d 21 before the morning feeding and ruminal fluid was collected same day via rumen cannula at 4 h post feeding. Metabolomics data were analyzed with MetaboAnalyst 6.0. We identified 2033 metabolites in rumen fluid and 627 in plasma. 22 altered (P ≤ 0.05) metabolites were identified between the SCFP and CON groups. The most notable upregulated metabolites in the SCFP group include pyruvic acid, 2-hydroxy-6-oxo-6-(2-hydroxyphenoxy)-hexa-2,4-dienoic acid, and diphenol glucuronide. The most downregulated metabolites in the SCFP group were glycyl-glycine, N-acetyl-6-pyruvoyltetrahydropterin and trans-1,2-dihydrobenzene-1,2-diol. Pathway enrichment analysis showed that SCFP supplementation significantly (P ≤ 0.05) impacted five metabolic pathways; dioxin degradation, xylene degradation, terpenoid backbone biosynthesis, C5-branched dibasic acid metabolism, and the pentose phosphate pathway. A total of 12,138 negative and 11,816 positive correlations were identified between rumen and plasma metabolites. Among these, correlations relevant to rumen fermentation, energy and amino acid metabolism were reported. Ruminal L-arginine shows a positive correlation with plasma glutamine (|r| = 0.63, P < 0.05) while ruminal asparagine was negatively correlated with plasma methionine (|r| = - 0.69, P < 0.05). A positive correlation was observed between rumen acetic and propionic acid with plasma pyruvic acid (|r| = 0.64 and 0.74, respectively; P < 0.05). A negative correlation was observed between linoleic acid in the rumen and alanine in the plasma (|r| = - 0.66, P < 0.05). Ruminal serotonin was negatively correlated with plasma isoleucine (|r| = - 0.68, P < 0.05), leucine (|r| = - 0.67, P < 0.05), and threonine (|r| = - 0.77, P < 0.05). Our results suggest that SCFP supplementation modulates rumen fermentation and systemic metabolism by enhancing amino acid metabolism, and energy utilization. Also, only pyruvate was significantly affected within the toxin degradation pathways, suggesting a potential metabolic response rather than direct toxin breakdown. Since many ruminal metabolites were highly correlated with plasma metabolites, future studies may explore correlation between blood and rumen microbial metabolites.
DOI: 10.1093/jas/skaf300.673
Language: English
Open Access status: bronze
OpenAlex ID: W4414831236
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