Work

TRAF2 and NCK interacting kinase: a novel regulator of integrin αIIbβ3 signaling in platelets

Year: 2025

Type: article

Abstract: Background: TRAF2 and NCK interacting kinase (TNIK) is a serine/threonine kinase member of the germinal center kinase family. Previously, in other cell types, TNIK has been shown to interact with key cytoskeletal regulatory proteins, to regulate F-actin distribution, cell polarization, and neuronal cell arborization. Proteomic studies have demonstrated TNIK is present in platelets; however, no further studies have explored if TNIK plays a role in the regulation of platelet function. Objectives: We sought to investigate the distribution of TNIK in platelets and characterize a potential role for TNIK in platelet function. Methods: The importance of platelet TNIK was explored using 2 structurally distinct TNIK inhibitors (KY-05009 and NCB-0846) in aggregometry, assays of granule secretion, calcium mobilization and thrombus formation, and using confocal microscopy and co-immunoprecipitation. Results: through direct associations with focal adhesion kinase, paxillin, kindlin-3, filamin A, Rap2a, gelsolin and actin. Conclusion: to assess TNIK's potential as a novel antithrombotic target.

Source: Research and Practice in Thrombosis and Haemostasis

DOI: 10.1016/j.rpth.2025.103204

Authors: Lily J. Bull, Scott Spencer, Ronit Bedi, Rebecca Lewis, Lisa-Marie Holbrook

Institution: University of Surrey

Language: English

Topic: Platelet Disorders and Treatments

Subfield: Hematology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,250