In vitro tetraploid induction of the gooseberry (Ribes glossularia L.)
Year: 2025
Type: article
Abstract: Polyploidisation is a tool widely used in breeding programs of many crop plants. After doubling the number of chromosomes, favorable traits are very often enhanced due to the increased dose of genes determining the desired characteristics. This study aimed to develop an in vitro polyploidisation method and obtain tetraploids of gooseberries. For this purpose, in vitro cultures of five gooseberry genotypes were initiated and the media for their multiplication were optimized. The shoots of gooseberry 'Captivator', 'Invicta', and 'Macurines' were cultured on Murashige and Skoog (1962) (MS) medium with 0.5 mg l-1 meta-topolin (mT), 0.1 mg l-1 gibberellic acid (GA3), 0.1 mg l-1 indole-3-acetic (IAA), 30 g l-1 and those of 'White Triumph' the breeding line AGR9 on a Lloyd and McCrown (1981) (WPM) 0.2 mg l-1 mT, 0.1 mg l-1 GA3, 0.1 mg l-1 IAA. For polyploidisation, the shoot explants were incubated in the dark for two weeks on multiplication media appropriate for the cultivars, containing one of the following antimitotic agents: colchicine, trifluralin, oryzalin or amiprophos methyl. The strongest phytotoxic effects were observed for oryzalin and trifluralin (all the explants died). Tetraploids that survived were obtained for two genotypes, four in 'White Triumph' and three in AGR9. The polyploidisation efficiency was 1.7% and 1.9% in 'White Triumph' and AGR9, respectively. Compared to the diploid plants, the tetraploids had a lower multiplication rate and the number of shoots useful for rooting. It is the first report of obtaining gooseberry tetraploids.
Source: Scientia Horticulturae
Institution: Instytut Ogrodnictwa – Państwowy Instytut Badawczy
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $3,000
Award: G2639928175
OpenAlex ID: W4414872937