Year: 2025
Type: article
Abstract: High Resolution Image Download MS PowerPoint Slide Quorum sensing (QS) is a cell-to-cell signaling system that takes place at a key concentration (quorum) of signal molecules and via a peculiar signaling pathway. Both bacteria and yeasts possess QS mechanisms, mediated by specific molecules (farnesol, tyrosol, 2-phenylethanol, tryptophol) in yeasts, and N -acylhomoserine lactones (AHLs) and modified oligopeptides in bacteria. Here, we report the first chemical evidence of bacterial QS activity in yeast Saccharomyces cerevisiae (OS3 and V5 strains) by UPLC-MS/MS identification of N -octanoyl- and N -decanoyl-L-homoserine lactones in cell-free culture media extracts. The AHLs' presence was unexpected, as they are produced exclusively by bacteria. Tyrosol, a yeast signal molecule, was identified and quantified by NMR analysis. Metataxonomic analysis suggested the existence inside S. cerevisiae cells of bacteria, including Firmicutes, Bacteroidota, and Proteobacteria . Our study paves the way for investigations into bacterial detection within S. cerevisiae cells and their role in biotechnological performance in the food fermentation fields.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
OpenAlex ID: W4414914932