Work

Establishment of a Modified Ferric Chloride-Induced Canine Superficial Femoral Artery Thrombosis Model

Year: 2025

Type: article

Abstract: High Resolution Image Download MS PowerPoint Slide Ferric chloride (FeCl 3 ) is a common agent for inducing thrombosis in laboratory animals. However, there is no sufficient data regarding the conditions of model preparation in dogs. We aimed to identify the optimal concentration and solvent of FeCl 3 for inducing canine superficial femoral artery thrombosis. We applied 30, 40, or 50% FeCl 3 in 1 M hydrochloric acid or water topically on the superficial femoral artery for 30 min to induce thrombosis, respectively, with the distal end of the exposed artery clipped for 15 min. Then the thrombus size and blood flow were assessed by the High-Resolution Ultrasonic Photoacoustic Multimode Imaging System, and pathological changes were evaluated with HE staining. Regardless of the concentration or solvent, FeCl 3 solutions successfully induced stable and dense thrombi. At day 28, 30% FeCl 3 in 1 M HCl demonstrated greater peak systolic velocity (PSV) ratios of normal and recanalization rates than 40% FeCl 3 in 1 M HCl. Except for day 14, 40% FeCl 3 in H 2 O had lower PSV ratios and recanalization rates compared to 50% FeCl 3 in H 2 O. The 40% FeCl 3 in H 2 O demonstrated greater blood flow recovery than the 40% FeCl 3 in 1 M HCl. Compared to the water solution, the acidic solution caused more pronounced endothelial cell damage, with no differences between varying concentrations in the same solvent. FeCl 3 application combined with blood flow blockage effectively induced stable thrombi in the superficial femoral arteries of dogs. Hydrochloric acid solutions demonstrated better thrombus formation efficacy than water.

Source: ACS Omega

DOI: 10.1021/acsomega.5c05177