Year: 2025
Type: article
Abstract: The lateral organization of voltage-dependent calcium channels in lipid membranes is crucial for their function. To visualize and quantify their nanoscale arrangement, we labeled calcium channels via the HaloTag strategy and imaged them with STED microscopy in live cells and supported plasma membranes derived from giant plasma membrane vesicles. The latter further improved lateral resolution and allowed quantification of protein density and the number of channels within a cluster.
Source: BIOspektrum
Author: Claudia Steinem
Institution: University of Göttingen
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $3,190
OpenAlex ID: W4414924780