Work

Molecular mechanisms of improving high internal phase emulsions stability of soy protein isolate and konjac glucomannan complexes fabricated with dynamic ultra-high pressure

Year: 2025

Type: article

Abstract: This study investigates the effectiveness and underlying mechanisms of dynamic ultra-high pressure (DUHP)-modified soy protein isolate (SPI) and konjac glucomannan (KGM) complexes in stabilizing high internal phase emulsions (HIPEs). The DUHP treatment of SPI enhanced its molecular structure, exposing hydrophobic amino acids that facilitated the formation of a stable non-covalent complex with KGM. The stability of these complexes primarily arises from the synergistic interactions of hydrogen bonds and van der Waals forces. The interaction between DUHP-treated SPI and KGM was significantly improved, leading to HIPEs stabilized by 0.4% KGM exhibiting smaller droplet sizes and superior emulsifying properties. The enhanced interfacial adsorption capacity of these complexes enabled a more uniform and effective distribution at the oil-water interface, which significantly increased the viscoelasticity of the emulsion. This improvement helps to prevent droplet aggregation and merging while also enhancing thermal stability. Furthermore, the DUHP treatment promoted non-covalent interactions between SPI and KGM, enhancing the interfacial activity of the complexes and facilitating the formation of a robust three-dimensional network structure. This structural enhancement contributes to the overall stability of HIPEs. The findings of this study offer valuable insights into the use of DUHP technology as a means to improve the stability of HIPEs. • DUHP significantly enhanced the interactions between SPI and KGM. • The DH-SPI-KGM complexes were formed through hydrogen bonds and van der Waals forces. • The HIPEs achieved optimal stability at 0.4% KGM concentration.

Source: LWT

DOI: 10.1016/j.lwt.2025.118593

Authors: Chang-Yue Ma, Chen Wang, Xu-Mei Wang, Jun Liu, Zong-Cai Tu, Yan-Hong Shao

Institutions: Jiangxi Normal University, Nanchang University, State Key Laboratory of Food Science and Technology

Language: English

Topic: Proteins in Food Systems

Subfield: Food Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,240

Funder: National Natural Science Foundation of China