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Molecular Detection and Study of some Virulence Factor Genes Among Pseudomonas aeruginosa Isolated from Different Human Clinical Specimens

Year: 2025
Type: article
Abstract: Introduction: Pseudomonas aeruginosa is an opportunistic pathogen causing various healthcare-associated acute and chronic infections. The multifactorial virulence of the ubiquitous opportunistic pathogen Pseudomonas aeruginosa has been linked to various virulence factors. Objective: Isolate and identify Pseudomonas auroginosa in different types of human clinical specimens, and evaluate the prevalence of four virulence genes (toxA, exoS, oprL, and oprI) in these isolates. Methods: The present study examined ninety-seven P. aeruginosa isolates from various types of patient clinical specimens, including wound swabs (n =33), ear discharge samples (n =28), urine samples (n =22) and burn swabs (n =14), during the period from September 2023 to February 2024 in Babylon governorate, Iraq. All colonies of P. aeruginosa were sub-cultured onto the bacterial culture, and then confirmed as P. aeruginosa by using microbiological tests and the API 20E test. Next, the PCR technique was carried out to screen the four virulence genes (toxA, exoS, oprL, and oprI).Results: P. aeruginosa was the most frequently isolated organism from wounds, followed by the ear, urine, and burns. The urban community shows a higher prevalence (62.9%) than the rural community (37.1%). The oprI and oprL genes were the most common genes detected among all tested isolates 97 (100%), while the toxA gene was detected in 79 (81.4%) isolates, and the exoS gene was detected in 68 (70.1%) isolates. Conversely, the results indicated that oprI, oprL, toxA, and exoS genes were the most commonly found genes in P. aeruginosa isolates from wound swabs, followed by ear discharge samples, urine samples, and burn swabs. Conclusion: PCR was empirically found to be the most optimal technique to detect and differentiate the virulence factor genes of Pseudomonas aeruginosa isolates that are involved in human infective diseases. How to cite this article:Rajendran R, Baruah K, Anusree S B, Sasi M S. Entomological Surveillance and Risk Assessment for Aedes-borne diseases in the Premises of Medical College Campus Institutions in Thiruvananthapuram Municipal Corporation. J Commun Dis. 2025;57(3):14-24. DOI: https://doi.org/10.24321/0019.5138.202564
DOI: 10.24321/0019.5138.202564
Language: English
Open Access status: hybrid
OpenAlex ID: W4414928368
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