Development of an analytical method based on derivatisation solid-phase microextraction combined with gas chromatography-mass spectrometry for the determination of bisphenol analogues in environmental waters
Year: 2025
Type: article
Abstract: • Efficient SPME-GC-MS method for nine bisphenols in environmental waters • Low cost in-vial acetylation enhancing analytical response without contamination • Multivariate optimisation of extraction parameters by experimental design • Reliable and sustainable approach with minimal solvent use and waste generation • Real world applicability with detection of target bisphenols in diverse waters Bisphenol analogues, including bisphenol A (BPA), are pervasive environmental contaminants known for their endocrine-disrupting properties. With the increasing use of alternative bisphenols due to regulatory restrictions on BPA, monitoring their presence in aquatic systems is critical. However, their low concentrations in environmental matrices complicate their detection. This study presents the optimisation using an experimental design of a solid phase-microextraction (SPME) method, coupled with gas chromatography-mass spectrometry (GC-MS), for the determination of nine bisphenols analogues (BPA, BPAF, BPF, BPE, BPG, BPB, BPZ, BPZ, and BPC2) in drinking water, environmental waters, and wastewater samples. Acetylation was used as a derivatisation technique to improve the analytes’ volatility and stability. The optimized method compromises the extraction of 15 mL of water by DVB/CAR/PDMS fibre in direction immersion mode adding 50 µL of acetic anhydride. The temperature was 100 °C and the extraction time 30 min. Method validation demonstrated excellent linearity (R 2 ˃ 0.999), low detection limits in the low µg L 1 range, and general recoveries above 80 % in real samples. The application to 19 water samples revealed the presence of seven bisphenols analogues reaching up to 3.4 μg L −1 , with BPE detected at the highest concentrations and BPA as the most frequently detected compound. This method provides a sensitive, efficient, and environmentally sustainable approach for the analysis of bisphenols in complex water matrices, aligning with green chemistry principles.
Source: Journal of Chromatography A
Institutions: Universidade de Santiago de Compostela, University of Bologna
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $4,040
Awards: G1405648912, G3616646022, G7125209824
OpenAlex ID: W4414939157