Year: 2025
Type: article
Abstract: Objective: Hepatocellular carcinoma (HCC) remains a significant global health challenge with limited effective therapies, necessitating the identification of novel therapeutic agents. Fig seed oil (FSO), rich in bioactive compounds, is being explored for its health benefits, including potential anti-cancer effects. This study investigated the cytotoxic, pro-apoptotic (via the mitochondrial pathway), and oxidative stress-modulating effects of FSO on HepG2 cells. Material and Method: HepG2 cells were treated with FSO (0.1-1% v/v). Cell viability was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay over 24, 48, and 72 hours. Oxidative stress markers, including Total Antioxidant Status (TAS), Total Oxidant Status (TOS), and Oxidative Stress Index (OSI), were measured by ELISA. Key apoptotic proteins (Bax, Bcl-2, cytochrome c, cleaved caspase-9, and cleaved caspase-3) were evaluated using Western blot analysis. Results: FSO demonstrated dose-dependent cytotoxicity against HepG2 cells, with an IC50 of 0.45% v/v at 72 hours. Treatment with 0.5% v/v FSO significantly improved cellular oxidative status by increasing TAS (1.8±0.2 vs 1.2±0.1 mmol Trolox eq/L, p
Source: SDÜ Tıp Fakültesi Dergisi
Author: Adem Yavaş
Institution: Adnan Menderes University
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
Funder: Adnan Menderes Üniversitesi
OpenAlex ID: W4414948680