Myeloid landscape profiling identifies DLBCL-specific suppressive macrophages colocalized with blood endothelial cells
Year: 2025
Type: article
Study design: Observational Study
Abstract: ABSTRACT: Diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) are the 2 most common B-cell lymphomas and are characterized by a dynamic crosstalk between tumor B cells and a heterogeneous, tumor-supportive microenvironment, such as immune, endothelial, and stromal components. Despite their recognized impact on the pathogenesis and prognosis of B-cell lymphoma, tumor-associated macrophages (TAM) have not been extensively explored in these diseases. In this study, we investigated mononuclear phagocyte (MNP) heterogeneity at the single-cell level and the activation profile of MNP, stromal, and endothelial compartments, in B-cell lymphoma lymph nodes compared to reactive secondary lymphoid organs. This was achieved using a combination of mass cytometry, single-cell RNA sequencing, in silico and spatial imaging approaches. Our findings revealed a lymphoma-specific pattern of TAM and blood endothelial cell (BEC) coactivation. Furthermore, we identified in DLBCL a spatial interaction between Annexin A1 (ANXA1)-expressing BEC and formyl-peptide receptor (FPR1/2) and S100A9-expressing monocytes/macrophages. This crosstalk is associated with an immunosuppressive tumor microenvironment and an adverse prognosis in patients with DLBCL.
Source: Blood Advances
Authors: Juliette Ferrant, Simon Le Gallou, Francine Padonou, Charlotte Dubec-Fleury, Simon Leonard, Ilenia Papa, Charles Brottier, Céline Pangault, Anne Barthel, Vincent Launay, Guillaume Manson, Bénédicte Hoareau, Laurent Deleurme, Céline Monvoisin, Benoit Albaud, Margaret A. Shipp, Nathalie Van Acker, Camille Laurent, Francisco Llamas Gutierrez, Henri-Alexandre Michaud, Nathalie Bonnefoy, Thierry Pécot, Karin Tarte, Mikael Roussel
Institutions: Inserm, Centre Hospitalier Universitaire de Rennes, Microenvironment and B-cells: Immunopathology, Cell, Differentiation and Cancer, Université Rennes 1, Université de Rennes, Hôpital Pontchaillou, Centre Hospitalier de Bretagne Sud, Centre Hospitalier de Saint-Brieuc, Sorbonne Université, Production et analyse de données en sciences de la vie et en santé, Centre National de la Recherche Scientifique, Structure Fédérative de Recherche en Biologie et Santé de Rennes, Université Paris Sciences et Lettres, Institut Curie, Dana-Farber Cancer Institute, Centre Hospitalier Universitaire de Toulouse, Institut universitaire du cancer de Toulouse Oncopole, Université de Montpellier, Institut de Recherche en Cancérologie de Montpellier, Institut Regional du Cancer de Montpellier
Language: English
Topic: Immune cells in cancer
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,800
OpenAlex ID: W4414954357