Year: 2025
Type: article
Abstract: Conventional enzymes immobilization strategies within metal-organic frameworks (MOF) typically require solvents and energy, resulting in solvent residues, environmental pollution, and high energy consumption. In this work, we present a solvent- and energy-free solid-state strategy for synthesizing enzyme-zeolitic imidazolate framework (ZIF) biocomposites. The nucleation of enzyme-ZIF is driven by acid-base interactions through the directly mixing of enzymes with ZIF precursors. The resulting biocomposites exhibit up to a four-fold enhancement in enzymatic activity compared to liquid-phase methods and a 1000-fold increase compared to free enzymes. This enhancement is attributed to the ZIF's multiphase crystalline structure, larger pore size, significantly higher surface area, and greater total pore volume, as well as a more favorable enzyme conformation (e.g., an open active-site pocket) achieved during synthesis followed by immobilization. Additionally, the synthesized enzyme-ZIF composites display excellent stability against organic solvents, proteolytic enzymes, and high temperatures, along with outstanding recyclability. This simple and sustainable approach to biocatalytic ZIF synthesis paves the way for the development of green, efficient, and robust biocatalysts for industrial applications.
Authors: Zefang Yu, Bernt Johannessen, Ming Zhang, Hongyu Shi, Yanhan Wang, Yu Wang, Jun Ge, Kang Liang, Jieying Liang
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $5,510
Funders: Australian Nuclear Science and Technology Organisation, Analytical Center for the Government of the Russian Federation, Australian Synchrotron, University of New South Wales, Tsinghua University, Medical Research Council, Australian Research Council, National Health and Medical Research Council
OpenAlex ID: W4414967694