Comprehensive proteomic profile in newly diagnosed patients with inflammatory bowel disease: identification of potential biomarkers
Year: 2025
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: Identifying proteomic signatures in treatment-naïve individuals newly diagnosed with inflammatory bowel disease (IBD) may provide insights into the underlying pathophysiological mechanisms of the disease and aid in distinguishing Crohn's disease (CD) from ulcerative colitis (UC). DESIGN: In the discovery phase, label-free quantitative proteomics was performed to analyze proteomic profiles in serum extracellular vesicles (EVs), serum, urine, and intestinal tissue from 100 newly diagnosed IBD patients (50 CD and 50 UC), and 51 healthy controls (HC). Serum candidate biomarkers were validated using ELISA in a separate subset cohort (87 CD, 134 UC, and 99 HC), and immunohistochemistry was performed on biopsies from the discovery cohort to confirm findings. RESULTS: We identified 419 proteins in serum EVs, 468 in serum, 683 in urine, and 2603 in intestinal tissue. ELISA results showed lower levels of TTR and APOC3 and higher levels of ATRN in UC patients compared to HC. Similarly, CD patients showed lower TTR and higher ATRN levels compared to HC. Moreover, serum protein S10A9 was differentially upregulated in CD vs UC. Immunohistochemistry revealed increased PRDX4 and AZU1 expression in the ileum of CD patients, whereas AOFB expression was lower in the ileum of CD and in the left colon of both CD and UC compared to HC. CONCLUSION: This comprehensive proteomic study has identified a set of proteins differentially expressed in IBD, which may contribute to a better understanding of its mechanisms and hold promise as candidate biomarkers. Although these findings are preliminary, they warrant further investigation to evaluate their diagnostic and therapeutic relevance.
Source: Journal of Crohn s and Colitis
Authors: Montse Baldán-Martín, Ibon Iloro, Mikel Azkargorta, Irene Soleto, Macarena Orejudo, Cristina Ramirez, Jorge Mercado, Fabio Suárez-Trujillo, Cristina Sánchez-Sánchez, Ana Garre, Sabino Riestra, Montserrat Rivero, Ana Gutiérrez, Iago Rodríguez-Lago, Luis Fernández-Salazar, Daniel Ceballos, José Manuel Benítez, Mariam Aguas, Iria Bastón-Rey, Fernando Bermejo, María José Casanova, Rufo H Lorente-Poyatos, Yolanda Ber, Daniel Ginard, María Esteve, Ruth De Francisco, María José García, Rubén Francés, Jose Luis Cabriada, Pilar Soto, Pilar Nos, Manuel Barreiro-de Acosta, Iván Guerra, Daniel Hervías Cruz, Manuel Domínguez Cajal, Vanesa Royo, Montserrat Aceituno, Noa B Martín-Cófreces, Félix Elortza, Javier P Gisbert, María Chaparro
Institutions: Hospital Universitario de La Princesa, Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas, Instituto de Investigación Sanitaria del Hospital Universitario de La Princesa, Universidad Autónoma de Madrid, CIC bioGUNE, Hospital Universitario Central de Asturias, Instituto de Investigación Sanitaria del Principado de Asturias, Marqués de Valdecilla University Hospital, Instituto de Investigación Marqués de Valdecilla, Instituto de Salud Carlos III, Hospital General Universitario de Alicante Doctor Balmis, Instituto de Investigación Sanitaria y Biomédica de Alicante (ISABIAL), Hospital de Galdakao, Hospital Clínico Universitario de Valladolid, Hospital Universitario de Gran Canaria Doctor Negrín, Instituto Maimónides de Investigación Biomédica de Córdoba, Hospital Universitario Reina Sofía, Hospital Universitari i Politècnic La Fe, Complejo Hospitalario Universitario de Santiago, Hospital Universitario de Fuenlabrada, Hospital General Universitario de Ciudad Real, Hospital General San Jorge, Hospital Universitario Son Espases, University Hospital Mútua de Terrassa, Universitat de Miguel Hernández d'Elx, Centro de Investigación en Red en Enfermedades Cardiovasculares, Centro de Investigación Biomédica en Red
Language: English
Topic: Inflammatory Bowel Disease
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W4414971989