Work

Indole-3-carboxamide alleviates LPS-induced endometritis through suppressing ferroptosis and inflammation via regulating Aryl hydrocarbon receptor

Year: 2025

Type: article

Abstract: Purpose: Indole-3-carboxamide (I3A), a derivative of tryptophan indole, has been reported to have anti-inflammatory role. This study aims to investigate the effects of I3A on LPS-induced endometritis in mice. Methods: The mice endometritis model was established and I3A was administered to mice orally (150 mg/kg/day) for two days. TNF-α and IL-1β production were analyzed by ELISA. The protein expression was measured by western blot. The pathological changes of mouse uterine tissue were observed by H&E staining. Results: The experimental results showed I3A significantly alleviated LPS-induced uterine pathological injury. I3A treatment obviously attenuated LPS-induced MPO activity, TNF-α and IL-1β production. I3A also inhibited LPS-induced ferroptosis and NF-κB activation. Furthermore, I3A could up-regulate the expression of AhR and SLC7A11. The protective role of I3A on LPS-induced endometritis was reversed by AhR inhibitor CH223191. Conclusion: In conclusion, I3A inhibits LPS-induced endometritis in mice by attenuating inflammation and ferroptosis via the AhR-SLC7A11 signaling pathway.

Source: Frontiers in Cellular and Infection Microbiology

DOI: 10.3389/fcimb.2025.1649801

Authors: Feng Chen, Rujin Cheng, Xiangyang Chen, Zhiheng Guo

Institution: First Hospital of Jilin University

Language: English

Topic: Ferroptosis and cancer prognosis

Subfield: Pulmonary and Respiratory Medicine

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,801