Work

Investigating Disparities in Metabolite Concentrations Using Gas Chromatography Mass Spectroscopy Among Patients With Gestational Diabetes Mellitus and in Healthy Control Participants

Year: 2025

Type: article

Abstract: Objective: Metabolomics has been a promising strategy in recent years for understanding the intricate metabolic changes linked to diseases like gestational diabetes mellitus (GDM). Gas chromatography-mass spectrometry (GC-MS) has been used as analytical method in this study to explore the function of metabolomics in comprehending the underlying biochemical alterations in GDM. Methods: One hundred subjects were enrolled and divided into two groups, Group A with healthy control participants (HCPs) and Group B with GDM patients. Both groups’ plasma samples were taken, and concentrations of amino acid metabolites were evaluated using GC-MS analysis. Results: Leucine and Tyrosine levels were low in HCP, that is, (1.79±0.15 μM and 0.25±0.06 μM), whereas levels were high in GDM patients, that is, (23.58±1.73 μM and 0.466±0.015 μM), respectively. Similarly, tryptophan and histidine levels were low in HCP, that is, (0.41±0.10 μM and 0.271±0.072 μM), and levels were high in GDM patients, that is, (0.871±0.105 μM and 1.916±0.340 μM), respectively. Methionine and Phenylalanine levels were high in HCP (1.063±0.161 and 0.642±0.035), and levels were low in GDM patients (0.765±0.103 and 0.459±0.056), respectively. Conclusion: The findings of the current study suggested that GDM is associated with an increase in tyrosine, tryptophan, leucine, and histidine levels and a decrease in methionine and phenylalanine levels. Therefore, these amino acids could serve as a diagnostic biomarker and supplementation tool (methionine and phenylalanine) as a management strategy in GDM.

Source: Asian Journal of Pharmaceutical and Clinical Research

DOI: 10.22159/ajpcr.2025v18i10.55487

Authors: SAPNA BHARDWAJ, CHIRAG PASRICHA, PREETPAL SINGH, PRATIMA KUMARI, RUPINDER KAUR, RAVINDER SINGH

Institution: Chitkara University

Language: English

Topic: Metabolomics and Mass Spectrometry Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: diamond

Funder: Chitkara University