Advancing the Development of Subcutaneous Glucose Biosensors: Cargo‐Carrying Adhesive Biosensor Systems (CABs)
Year: 2025
Type: article
Abstract: ABSTRACT The development of an injectable, subcutaneous glucose biosensor may be advanced by utilizing optical glucose sensing assays. However, this requires a strategy to effectively house small‐sized assay molecules. Herein, “CABs” or cargo‐carrying adhesive biosensors were constructed via the electrostatic adhesion of a hollow rod membrane (whose cavity could store a liquid optical‐assay) and hydrogel caps. For the CAB ‘wall’, the hollow rod leveraged a comb double network (DN) design previously shown to limit biofouling and reduce mesh size. To regulate the mesh size, poly(AMPS)‐methacrylate (PAMPS n ‐MA) comb macromers were incorporated into the 1st network. To improve electrostatic adhesivity, anionic 2‐acrylamido‐2‐methylpropane sulfonic acid (AMPS) was incorporated into the second network of the DN hydrogels at varying concentrations. For the CAB ‘cap’ with a cationic surface, a semi‐interpenetrating polymer network was formed, comprised of crosslinked polyampholyte and non‐crosslinked cationic polyelectrolyte. A CAB was constructed with a CAB wall based on a DN hydrogel composition shown to exhibit the requisite thermosensitivity, mechanical robustness, glucose diffusivity, low mesh size (4 nm < ξ < 7 nm), and adhesivity to the CAB cap. Using FITC‐dextran solutions, the CAB was shown to retain ∼90% of molecules of low hydrodynamic diameters ( D h ∼ 7 nm and D h ∼ 10 nm).
Source: Macromolecular Bioscience
Authors: Carolina I. Martinez, Theodore S. Ferrell, Varshitha M. Krishnan, Melissa A. Grunlan
Institution: Texas A&M University
Language: English
Subfield: Molecular Medicine
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,880
Funder: National Science Foundation
OpenAlex ID: W4415097312