Work

Rolling into the genome: linking mutations to cellular structure through label-free holographic cytometry

Year: 2025

Type: article

Abstract: Holo-tomographic flow cytometry for label-free phenotyping of suspended acute myeloid leukemia blasts is demonstrated. A concave segmentation algorithm is applied to 3D refractive index tomograms to quantify NPM1-mutation-associated cup-like nuclear morphologies, with virtual reality visualization offering engaging immersion. The method enables population-level detection of statistically significant shifts in 3D cell morphology, originally correlating phenotype with genotype.

Source: Light Science & Applications

DOI: 10.1038/s41377-025-02053-z

Author: Maciej Trusiak

Institution: Warsaw University of Technology

Language: English

Topic: Digital Holography and Microscopy

Subfield: Atomic and Molecular Physics, and Optics

Field: Physics and Astronomy

Domain: Physical Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,190