Work

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

Year: 2025

Type: article

Abstract: Enteropathogenic E. coli (EPEC) attaches to host intestinal epithelial cells, resulting in severe illness and diarrhoea. Different cell models have been used to study EPEC infection, but a direct comparison of infection of cell lines and primary cells is lacking. In this study, we compare EPEC infection in primary epithelial cells with HeLa cells. Jejunal organoid-derived cells contain differentiated intestinal epithelial cells and form a tight monolayer. Upon infection, they retain integrity over longer time periods than HeLa cells. Attachment of EPEC to host cells and innate immune response is strongly delayed in organoid-derived monolayers. These results indicate that host cell factors determine the outcome of infection. Future identification of these host factors will aid the development of new therapeutics.

Source: FEBS Letters

DOI: 10.1002/1873-3468.70182

Authors: Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld

Institutions: University of Würzburg, Technische Universität Berlin, Charité - Universitätsmedizin Berlin, Universitätsklinikum Würzburg

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,980