Work

Structural and Spectroscopic Insights into Catalytic Intermediates of a [NiFe]‐hydrogenase from Group 3

Year: 2025

Type: article

Abstract: production and are potential models for renewable energy catalysts. Here, the full redox landscape of a group 3 [NiFe]-hydrogenase from methanothermococcus thermolithotrophicus is elucidated, resembling group 1 enzymes. Structural and spectroscopic analyses reveal a catalytic-ready state with nickel seesaw coordination, enabling intermediate trapping and advancing mechanistic understanding of oxygen-sensitive [NiFe] enzymes.

Source: ChemBioChem

DOI: 10.1002/cbic.202500692

Authors: Marion Jespersen, Christian Lorent, Olivier N. Lemaire, Ingo Zebger, Tristan Wagner