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In vitro Assessment of Bio-agents and their Culture Filtrate for Sustainable Management of Rhizoctonia solani

Year: 2025

Type: article

Abstract: Rice is a vital cereal crop that forms the staple diet for more than half of the global population. It plays a critical role in food security and nutrition, particularly in Asia, where countries such as China, India, Indonesia and Bangladesh lead the way in cultivation and consumption but severely affected by Rhizoctonia solani causing heavy yield loss. This study investigated the in vitro efficacy of several bio-agents and their culture filtrates in suppressing the mycelial growth of Rhizoctonia solani. In the first experiment, direct applications of Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens and Bacillus subtilis significantly reduced fungal radial growth, with T. harzianum (68.37% inhibition) and T. viride (59.18% inhibition) showing the most potent antagonistic activity compared to the untreated control. In the second experiment, culture filtrates from various bio-agents (Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens and Bacillus subtilis) were evaluated. Results revealed that T4 and T9 produced the highest percent inhibition on the seventh day (82.95% and 78.18%, respectively), while other treatments showed moderate to low efficacy (13.37–59.45% inhibition). Statistical analysis (CD and SE) confirmed the significance of these differences among treatments in both experiments. The collective results indicate that both living bio-agents and their culture filtrates, particularly those derived from effective strains such as Trichoderma spp., offer strong potential for the biological control of R. solani, contributing to sustainable plant disease management strategies.

Source: International Journal of Plant & Soil Science

DOI: 10.9734/ijpss/2025/v37i105779

Authors: Saurabh Saini, S. K. Biswas, Anju Shukla, Prabha Siddharth, Girijesh Kumar Jaisval, Akash Kumar Kamal, Shivam Kumar, Utkarsh Upadhyay

Institutions: Chandra Shekhar Azad University of Agriculture and Technology, Acharya Narendra Deva University of Agriculture and Technology

Language: English

Topic: Plant-Microbe Interactions and Immunity

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDGs: Zero hunger, Good health and well-being

Open Access status: diamond

OpenAlex ID: W4415191759

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